HLS2 全自动超长DNA片段分选回收仪： 50kb-2Mb; 靶向长片段获取; 一步法提取线粒体DNA
• Sage Science 公司
位于美国马萨诸塞州，2010年成立，专注于核酸领域的样品制备
全球超过3000家客户的认可，国内装机数量400台左右
2万篇文献中应用，包括众多Nature、 Science、 Cell系列的文章
DNA片段筛选回收领域的行业金标准
• Sage HLS2 全自动超长DNA片段分选回收仪
整合了脉冲场电泳，直接从核酸样品中完成DNA超大片段的分选回收
回收范围：50kb-2Mb DNA;
用于Nanopore、华大、齐碳、普译等超长测序（Ultra long Sequencing）文库构建
用于CATCH技术获取的上百kb靶向克隆基因组片段回收
技术原理
产品特点
应用方向
典型案例
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案例1： Sage HLS2 用于超长测序（Ultra long Sequencing）文库构建，只保留50kb以上的DNA，提高Reads N50长度，降低测序成本
50kb High Pass超长基因组，去除50kb以下DNA |
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案例2： 经过SageHLS2自动化片段筛选，建库后Nanopore测序在单个cell上Reads N50可达143.3 kb
Reads N50可达143.3 kb（数据来源：希望组官方公众号） |
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发表文献
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1.Eveleigh, Robert JM, et al. "Finding an optimal sequencing strategy to detect short and long genetic variants in a human genome." bioRxiv (2025): 2025-05. 2.Liu, Xin, et al. "Telomere‐to‐telomere reference genome reveals subgenome divergence and large structural variants in Capsella bursa‐pastoris (Brassicaceae)." Biological Diversity 2.1 (2025): 28-38. 3.Fang, Zhenyu, et al. "Experimental insights into genome reconstruction driven by horizontal transfer of supernumerary chromosomes in Magnaporthe oryzae." New Phytologist 248.1 (2025): 140-156. 4.Yadav, Hemant Kumar, et al. "Telomere‐to‐telomere genome assembly of linseed (Linum usitatissimum L.) for functional genomics and accelerated genetic improvement." Plant Biotechnology Journal (2025). 5.Wen, Huaming, et al. "TRFill: synergistic use of HiFi and Hi-C sequencing enables accurate assembly of tandem repeats for population-level analysis." Genome biology 26.1 (2025): 227. 6.Xia, Zhiqiang, et al. "Pan-genome and haplotype map of cassava cultivars and wild ancestors provide insights into its adaptive evolution and domestication." Molecular Plant 18.6 (2025): 1047-1071. 7.Zhou, Bo, et al. "Resolving the 22q11. 2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints." Proceedings of the National Academy of Sciences 121.31 (2024): e2322834121. 8.Cai, Yudong, et al. "Genome sequencing of ‘Fuji’apple clonal varieties reveals genetic mechanism of the spur-type morphology." Nature communications 15.1 (2024): 10082. 9.Gao, Shenghan, et al. "The centromere landscapes of four karyotypically diverse Papaver species provide insights into chromosome evolution and speciation." Cell Genomics 4.8 (2024). 10.Yang, Chentao, et al. "The complete and fully-phased diploid genome of a male Han Chinese." Cell Research 33.10 (2023): 745-761. |
